A Eurogentec product line, distributed in Malaysia by Infenix Sdn Bhd.
What It Is
Eurogentec’s RNAi Oligonucleotides line covers three ready-to-order families: custom siRNA duplexes synthesised to your sequence, a catalogue of ready-to-use positive and negative siRNA controls, and custom miRNA mimics and inhibitors.
All are chemically synthesised RNA oligonucleotides made in-house by Kaneka Eurogentec in Seraing, Belgium, using phosphoramidite solid-phase synthesis, with free sequence design available from Eurogentec’s oligonucleotide design team. Products are supplied dried or lyophilised for research use, from a few nanomoles up to large custom quantities.
How It Works
A synthetic siRNA duplex is introduced into the cell, where the double-stranded RNA is recognised by the RISC complex. The sense strand is displaced, the target mRNA anneals to the antisense strand held by RISC, the mRNA is digested, and RISC is recycled for further rounds of silencing. Because the effect is sequence-specific, expression of one chosen gene is knocked down without a genomic edit.
miRNA mimics are double-stranded synthetic microRNAs that reproduce the action of endogenous miRNAs, while miRNA inhibitors are chemically modified antisense oligos that bind and neutralise a specific miRNA — giving gain- and loss-of-function control over the same regulatory pathway.
Key Specifications
| siRNA format | Standard 21-mer: 19 double-stranded RNA bases plus a 2-base single-stranded DNA 3′ overhang (usually dTdT). Total 21–27 bases |
| Synthesis scales | SePOP-purified: 7, 22, 60 or 200 nmol. HPLC-purified: 3, 12, 40 or 80 nmol. Larger scales on request |
| Purification | SePOP desalting, IEX-HPLC or RP-HPLC, or the “in vivo” process (size-exclusion desalting, sterile filtration, lyophilisation; production process endotoxin tested) |
| Quality control | Every custom siRNA production controlled by MALDI-TOF MS, included free for siRNA duplexes |
| Free design & guarantee | Submit your target gene and receive 3 siRNA candidate sequences. Order all 3 duplexes and Eurogentec guarantees at least one achieves a minimum of 80 % gene silencing |
| Backbones & modifications | RNA, LNA®, 2′O-Me RNA or 2′O-MOE RNA bases; optional phosphorothioate linkages. 5′: phosphate, 6-FAM, AlexaFluor, ATTO, biotin, Cy3, Cy5, fluorescein, HEX. 3′: 6-FAM, Cy3, Cy5, rhodamine, TAMRA, TEG-cholesteryl |
| Delivery & annealing | Delivered dried at room temperature in a 2 mL tube. Free annealing on request, shipped with free annealing buffer and RNase-free water. Single strands orderable separately |
| Storage | Dried oligos stable 18 months at ambient temperature; 24 months at −20 °C in solution. Resuspend in RNase-free buffer at 20–50 µM, in small aliquots |
siRNA Controls
Ready-to-use positive controls against LaminB1, Vimentin, NuMA, β-actin, Eg-5 and Cdk-1 (human), pGL2 and pGL3 luciferase (firefly) and GFP (jellyfish), plus a negative control with no homology to any known eukaryotic gene.
Supplied as 1 duplex at 5 nmol, IEX-HPLC purified, 100 % MALDI-TOF controlled, already annealed and shipped dried. Eurogentec states a delivery time of 5 working days for these catalogue items.
Why It Matters
The free design service carries a stated silencing guarantee: order all three designed duplexes for a target and at least one is guaranteed to reach a minimum of 80 % knockdown. That removes much of the trial-and-error cost of validating a new target.
The same synthesis platform then scales from a 3 nmol pilot duplex through to sterile-filtered, endotoxin-tested material, with stabilising chemistries such as LNA®, 2′O-Me, 2′O-MOE, phosphorothioate and cholesterol conjugation — so a laboratory does not need to change supplier as a project moves from cell culture towards in vivo work.
Applications
- Sequence-specific gene knockdown in transfected cell lines and primary cells
- Gene function studies
- Loss-of-function screening using multiple duplexes per target gene
- Validated negative and positive siRNA controls to monitor assay conditions
- miRNA gain-of-function studies using synthetic double-stranded mimics
- miRNA loss-of-function studies using chemically modified inhibitors
- Tracking uptake and localisation with dye-, biotin- or cholesterol-labelled RNAi oligos
- In vivo RNAi work using sterile-filtered, endotoxin-tested material
Enquiries
Infenix Sdn Bhd distributes Eurogentec in Malaysia. Call +6012 882 6283 / +6012 882 6289 or email enquiry@infenix.com.my.