A Eurogentec product line, distributed in Malaysia by Infenix Sdn Bhd.
What It Is
NGS Oligos are Eurogentec’s Next-Generation Sequencing grade custom oligonucleotides: single-stranded DNA sequences you specify, synthesised for use as sequencing adapters, index (barcode) oligos and library-preparation primers.
They are ordered per sequence at 20 to 85 bases, with a minimum of 10 nmol delivered dried in tubes, purified by RP-HPLC or RP-Cartridge Gold and 100 % checked by MALDI-TOF mass spectrometry. Optional 5′ Phosphate or 5′ Biotin-TEG modifications, phosphorothioate linkages and degenerate (wobble) bases are configurable at the point of order, with wobble bases carrying no additional cost.
How It Works
In a multiplexed NGS run, adapters carrying a short index sequence are ligated onto fragmented DNA, many samples are pooled into a single sequencing run, and reads are afterwards assigned back to their source sample by reading that index.
Eurogentec describes the problem this creates: adapter sequences added to fragmented nucleic acids need high purity with no by-products and low cross-contamination. Trace carry-over of one index oligo into another during manufacture surfaces as reads assigned to the wrong sample — what Eurogentec calls barcode misalignment — while truncated synthesis by-products yield adapters that ligate or sequence poorly.
The product addresses both failure modes through a dedicated production process holding cross-contamination below 0.1 %, HPLC or cartridge purification to strip synthesis by-products, and MALDI-TOF mass confirmation on 100 % of oligos.
Key Specifications
| Cross-contamination | Low cross contamination, <0.1 % |
| Length | 20 to 85 bases |
| Quantity | 10 nmol minimum delivered |
| Purification | RP-HPLC or RP-Cartridge Gold |
| Quality control | 100 % QC checked by MALDI-TOF MS; supplied with a technical data sheet |
| 5′ modifications | 5′ Phosphate or 5′ Biotin-TEG |
| Backbone option | Phosphodiester as standard; phosphorothioate bond available as an option |
| Wobble bases | Available at no additional cost, specified using IUB codes |
| Format & storage | Dried in tubes. Dried oligos stable 18 months at ambient temperature; 24 months at −20 °C once in solution |
Why It Matters
Index cross-contamination is a low-level, hard-to-diagnose failure. Carry-over between barcode oligos surfaces as reads assigned to the wrong sample across every library in the pool, and it is typically only caught after the data comes off the instrument — by which point the sequencing capacity has already been spent.
Eurogentec manufactures these oligos specifically against that failure mode: below 0.1 % cross-contamination, RP-HPLC or RP-Cartridge Gold purification, and MALDI-TOF QC on every oligo.
Applications
- NGS library preparation for DNA sequencing workflows
- Sequencing adapter oligos ligated onto fragmented nucleic acids
- Index and barcode oligos for sample multiplexing
- Highly multiplexed pooled runs where barcode misalignment must be minimised
- 5′ Phosphate adapters for ligation-dependent library construction
- 5′ Biotin-TEG oligos for streptavidin-based capture and pulldown
- Library amplification and indexing primers within the 20–85 base range
- Designs needing degenerate (wobble) positions or phosphorothioate linkages
Lead Time
Eurogentec’s published schedule gives 4–6 working days for RP-Cartridge Gold and 5–7 working days for HPLC, and states that these values are indicative. These are manufacturing lead times only — they are not a delivery commitment to Malaysia, as international transit and customs clearance are additional. Contact us for a delivery estimate on your order.
Enquiries
Infenix Sdn Bhd distributes Eurogentec in Malaysia. Call +6012 882 6283 / +6012 882 6289 or email enquiry@infenix.com.my.